It is a mosquitoborne, single positivestranded rna virus of the family flaviviridae. The genome of flaviviruses is approximately 11 000 bases long and they share common. Dengue diagnosis was one of the topics discussed at the adult dengue presentations. Denv is transmitted to humans by the mosquito vector aedes aegypti and is the most prevalent arbovirus in tropical and subtropical areas. This report further demonstrates the role of travellers as. Phylogenetic analysis identified identical sequences belonging to the cosmopolitan genotype of dengue virus type 2 in all samples sequenced, thus providing evidence for a probable dengue type 2 outbreak in the seychelles. In the last 50 years, dengue incidence has increased 30fold, worldwide. Nevertheless, scientists understanding of dengue virus may be simplistic, as rather than. Complete genome sequences of dengue virus type 2 epidemic. Dengue virus denv, fulllength genome, dengue virus type 1 denv1, genetic variants, dengue. A retrospective study found no evidence that yellow fever vaccination in dengueendemic areas increases risk for severe dengue fever. Whole genome sequencing and genetic variations in several dengue virus type 1 strains from unusual dengue epidemic of 2017 in vietnam.
The disease is caused by four serologically and genetically distinct viruses termed dengue virus denv 1, 2, 3 and 4. A group of 8 coinfected mosquitoes were placed in a vial with the nylon mesh side against the ears of an anesthetized balbc mouse. Associations between certain dengue virus genotypes with severe dengue outbreaks may point to strains with increased pathogenicity whereas association between dengue strains with unusual phenotypic traits may help identify the responsible viral genetic determinant. However, the number of patients with dengue fever has increased drastically during the current and largest outbreak, during januaryjuly 2017, when 90,000 patients were reported in. This months genome watch highlights how deep sequencing has provided insights into dengue virus evolution under different selection pressures. Similar strains of denv2 have repeatedly caused outbreaks in west africa 8. Molecular characterization of the viral structural gene of the.
Briefings in functional genomics advance access published january 10, 2017. Dengue virus net is the web resource for anyone interested in dengue. Global incidence of dengue has grown dramatically in recent decades. Common fungus helps dengue virus thrive in mosquitoes. Jan 10, 2017 genomics, proteomics and evolution of dengue virus article pdf available in briefings in functional genomics 164 january 2017 with 333 reads how we measure reads. Complete genome characterization of the 2017 dengue. Dengue is often a leading cause of illness in areas. We describe here the complete genome sequencing of the denv2 strain. Pdf antivirus effectiveness of ivermectin on dengue. A dengue outbreak abruptly occurred at the border of china, myanmar, and laos in june 2017. Nov 06, 2018 rna viruses usurp and reprogram host cells using short rna genomes.
Laboratory diagnosis is also important for case confirmation. The viral particle encapsulates a positive strand rna of 11 kb that constitutes the viral genome, with 5 and 3 untranslated regions utr and a 5 cap. Pdf the genome of a pathogenic organism possesses a specific order. This enveloped virus contains a positive singlestranded rna genome.
Utrs of dengue virus play an important role in the genome replication of rna wei et al. By november 3rd 2017, 1184 infected individuals were confirmed as ns1positivein xishuangbanna, a city located at the border. Dengue is a mosquitoborne infection that causes a severe flulike illness dengue fever, and sometimes a potentially lethal complication called dengue haemorrhagic fever. Pervasive tertiary structure in the dengue virus rna genome elizabeth a. The outbreak and severe dengue were attributed to the new dengue virus type 1 denv1 strain, which has since remained the predominant serotype in sri lanka. Genomic epidemiological characteristics of dengue fever in. The conserved molecular determinants of virulence in dengue virus.
Dec 07, 2017 a species of fungus that lives in the gut of some aedes aegypti mosquitoes increases the ability of dengue virus to survive in the insects, according to a study from researchers at johns hopkins. Eurosurveillance an outbreak of dengue virus denv type. Epidemiology investigation and phylogenetic analysis of circulating viruses revealed that at least three lineages of dengue virus serotype 2 denv2. Accurate and efficient diagnosis of dengue is important for clinical care, surveillance support, pathogenesis studies, and vaccine research. Dec 07, 2017 no dengue virus nonstructural proteins are encoded in the dengvaxia genome. Research paper insilico designing and testing of primers for.
Complete genome characterization of the 2017 dengue outbreak. Dengue virus has recently reemerged in the southern indian ocean islands, causing outbreaks in reunion island and the seychelles. In addition to the epidemic caused by denv, which has been described in many tropical countries, the introduction of chikv and zikv in these countries is a major public health concern. Pdf genomics, proteomics and evolution of dengue virus.
I n 2009, sri lanka experienced an outbreak of dengue. Dengue virus denv infection is responsible for the most significant mosquitoborne viral disease in the world today. A risk to anyone traveling to a tropical or subtropical region of the world where dengue virus is found. In june 2017, dengue virus type 2 infection was diagnosed in 2 travelers returned to japan from sri lanka, where the countrys largest dengue fever outbreak is ongoing. First complete genome sequences of dengue virus serotype 2. Genome variations and divergences also exist among zikv strains isolates. These results indicated that denv1 from the dengue epidemic 2017 in northern vietnam originated from the virus that caused the dengue outbreak during the 2007 to 2008 period in vietnam. The global incidence of dengue has grown dramatically in recent decades. During designing and selection of primers these complexities needs to be over ruled. Research paper insilico designing and testing of primers. Dengue virus pirates human platelets blood american. The 2017 dengue outbreak in the border areas around xishuangbanna was not caused by only one denv1 source strain, but belong to a single genotype. Dengue, one of the most devastating mosquitoborne viral diseases in humans, is a significant life threatening problem in india and other developing countries. More than 50% of the viruses in the genus flavivirus, family flaviviridae, cause disease in humans, including dengue, yellow fever, west nile and zika viruses.
As of april 2017, denv2 and denv3 have been isolated. In the present study, we determined the complete genome sequences of closely related clinical isolates of dengue virus type 2 circulating in the. Dengue viruses are spread to people through the bite of an infected aedes species ae. On june 19, 2017, a man in his early 50s sought care at the center hospital of the national center for global health and medicine, tokyo, japan, for fever, chills, headache, and mild joint pain. Dengue is among the most important mosquitoborne viral disease in the world.
We use a new highthroughput rna structure probing technology to identify rna. Author summary dengue denv, chikungunya chikv and zika zikv are considered major public health challenges. Dengue virus news, articles the scientist magazine. Dengue and severe dengue world health organization. Phylogenetic analysis indicated that the dengue virus genome sequence in this case is highly homologous with recent strains in africa, especially from the 2016, 1986, and 1983 outbreaks in burkina faso. Zika virus genome biology and molecular pathogenesis.
Dengue virus type 2 in travelers returning to japan from. Dengue virus is one of the most widespread mosquitoborne viruses worldwide, with an estimated annual infection rate around 390 million. Global incidence of dengue has grown dramatically in. Genomewide mutagenesis of dengue virus reveals plasticity of. In order to make patient management and surveillance. Dengue is common in more than 100 countries around the world. Since the dengue virus was first isolated in 1943, studies to understand the pathogenesis of dengue has long been hindered by the lack of suitable experimental models. Complete genome sequence of a highly divergent dengue virus. Pdf fullgenome dengue virus sequencing in mosquito. The zikv genome evolved rapidly from the flavivirus genus and diverged from the members of this genus, even within the dengue virus cluster to which zikv belongs. Nakayama 1, akatsuki saito 1, akio egawa 1, tairyu sato 1, juthamas phadungsombat 3. Tak003 appears to avoid the safety issues seen with an existing vaccine, but experts say a longer evaluation is needed. These arboviruses are primarily transmitted by mosquitoes of the species ae. The present study is the first of its kind to describe complete genome sequence as well as genetic variants and phylogenetic analysis of denv1 associated with.
Clinical and laboratory diagnosis of dengue virus infection. Dengue virus and zika virus transmission by bites of coinfected aedes aegypti. While most infections are asymptomatic, infection can range from mild dengue fever df to severe forms of denv infection, formerly referred to as dengue hemorrhagic fever dhf and dengue shock syndrome dss 23,24. Jan 20, 2020 these results indicated that denv1 from the dengue epidemic 2017 in northern vietnam originated from the virus that caused the dengue outbreak during the 2007 to 2008 period in vietnam. Review peptides as therapeutic agents for dengue virus. Dengue virus comprises four serotypes labelled denv 1 to 4. These genome divergences might account for the uniqueness of zika disease. Molecular epidemiology of dengue viruses from complete genome sequences inauguraldissertation zur. Coinfection with zika virus zikv and dengue virus results.
Pdf antivirus effectiveness of ivermectin on dengue virus. Each year, an estimated 390 million people are infected. Citation pyke at, huang b, warrilow d, moore pr, mcmahon j, harrower b. Forty percent of the worlds population, about 3 billion people, live in areas with a risk of dengue. In the present study, we determined the complete genome sequences of closely related clinical isolates of dengue virus type 2 circulating in the seychelles in 2016 and reunion island in 2018. Despite the availability of cydtdv as a commercial vaccine, its longterm efficacy against all four dengue virus serotypes remains unsatisfactory. In the context of recent arbovirus epidemics, dengue fever is becoming a greater concern around the. Dengue denv and zika zikv viruses are clinically important members of the flaviviridae family with an 11 kb positive strand rna genome that folds to enable virus function. The recent emergence and reemergence of viral infections transmitted by vectorszika, chikungunya, dengue, japanese encephalitis, west nile, yellow fever and othersis a cause for international concern.
Full genome dengue virus sequencing in mosquito saliva shows lack of convergent positive selection during transmission by aedes aegypti november 2017 virus evolution 32. Five serotypes of the virus have been found, all of which can cause the full spectrum of disease. Multiple lineages of dengue virus serotype 2 cosmopolitan. Fulllength genome and molecular characterization of dengue virus. Eurosurveillance an outbreak of dengue virus denv type 2. Molecular epidemiology of dengue viruses from complete genome. Isolates of dengue virus serotype 2 denv2 were recovered from a female resident of the solomon islands in 2016 and another female patient who had traveled from vanuatu to australia in 2017. The virus responsible for causing dengue, is called dengue virus denv. The authors show that platelets synthesize ns1 protein and. Dengue virus type 2 in travelers returning to japan from sri.
Jun 14, 2017 serotyping dengue viruses using laboratory materials. Because dengvaxia has been associated with ade, the philippines has withdrawn. A retrospective study found no evidence that yellow fever vaccination in dengue endemic areas increases risk for severe dengue fever. In this study, we performed phylogenetic, recombinant, and nucleotide variation analyses of 114 complete genome sequences of dengue virus serotypes 14 denv14 collected from 20 to 2017 in 18 of 21 cities of guangdong. Like other flaviviruses, its genome comprises a single strand of positivesense rna encoding 3 structural and 7 nonstructural ns proteins. Rna viruses encode the information required for their replication in both their primary sequences and higherorder structures formed when the rna genome strand folds back on itself, but the extent of higherorder structure has remained unclear. This is responsible for the heavy burden on the control of dengue, and raises the concern about whether dengue outbreaks have become endemic in guangdong. A computational method for the identification of dengue. To verify the causative agent, complete genome information was obtained through pcr and sequencing based on the viral rnas extracted from patient samples.
Considering the challenges of an appropriate primer design for pcr based sequencing of whole genome of dengue virus subtypes, manyof the pcrbased whole genome sequencing methods, as reported in the literature. Dengue virus infection was diagnosed in six israeli travellers returning from the seychelles in april 2017. Nile virus wnv, yellow fever virus and the recently emerging zika virus zikv. Previous studies based on single e gene or few fulllength genome sequences were. In june 20, he had traveled to abidjan for business, and on june, 2017, he returned to japan. Using a panel of techniques, including a ribonucleoproteomic approach based on tagged protein affinity purification combined with nextgeneration sequencing, chazal et al. Virus pathogen database and analysis resource vipr.
The overall scheme for the sample processing and sequencing analysis of a dengue virus is shown in fig. The viral genomes resource is a collection of viral genomic sequences that is a part of the entrez genomes, which provides curated sequence data and annotations of complete genomes to the scientific community. Dengue virus denv is a major global pathogen that causes significant morbidity and. Using as examples zika, chikungunya and dengue, we summarise current knowledge on characteristics of the viruses and their transmission, clinical features, laboratory diagnosis, burden. It is a mosquito borne, single positivestranded rna virus of the family flaviviridae. Molecular epidemiology of dengue viruses from complete. There are four types of denv serotypes denv1, denv2, denv3 and denv4, meaning that it is possible to be infected four times. As scott halstead wrote, the substitution of yellow fever nonstructural proteins for those of dengue virus may have crippled dengvaxia protective t cell responses to dengue infections. Nevertheless, scientists understanding of dengue virus may be simplistic, as rather than distinct antigenic groups, a continuum appears to exist. Dengue virus 1 den1 was first discovered in japan in the 1940s.
All patients were clinically diagnosed as mild dengue. Unlike the other flaviviruses, there are 4 serotypes, referred to as denv14, that are genetically similar but antigenically. These results provide detailed evidence to elucidate the origin, circulation, and genetic evolution of denv in vietnam. Transposon mutagenesis of the zika virus genome highlights regions essential. Travelers, especially those previously affected by dengue fever, should take measures to avoid mosquito bites. Structure mapping of dengue and zika viruses reveals. Wed like to understand how you use our websites in order to improve them. In the 2017, a dengue epidemic occurred in hanoi in a short time. This study aims to explore the genetic characterization, potential source and. Dengue virus exported from cote divoire to japan, june 2017. Dengue hemorrhagic fever is an acute viral infection transmitted by mosquitoes. Pervasive tertiary structure in the dengue virus rna genome. Each denv has four or more genetic groups or genotypes.
Author summary in 1978, dengue fever was first reported in guangdong province, china, and this has been continuously prevalent in guangdong in recent years. The fda announced approval of dengvaxia vaccine for prevention of dengue disease caused by all dengue serotypes in people ages 916 who have laboratoryconfirmed prior infection and live in endemic areas. Here, we describe the first complete genome sequences of denv2 strains from vanuatu and the solomon islands. They belong to the genus flavivirus, family flaviviridae of which yellow fever virus is the type species, which contains approximately 53 viruses. The other three viruses, den2, den3, and den4, are closely related to den1, but are not recognized as the same virus by. A group of 8 coinfected mosquitoes were placed in a vial with the nylon mesh side against the.
It is a singlestranded rna virus transmitted by aedes aegypti or aedes albopictus mosquitoes in tropical regions of the world. Dengue virus denv infects approximately 400 million people annually. Whole genome sequencing and genetic variations in several. Dengue is an arthropod borne virus belongs to family flaviviridae and genus flavivirus dengue virus is transmitted between people by female mosquitoes ades aegypti and ades albopidus, which are found throughout the tropical and subtropical regions. Dengue is a disease caused by infection with one of the four dengue virus serotypes denv1, 2, 3, and 4.
1143 1300 1493 1400 1228 846 239 1512 173 915 590 97 1173 301 1078 362 352 57 1238 1029 134 340 1180 756 1426 87 1355 1271 214 941 665 1178 1482 1166 288 911 388 534 235 218 595 21 1348 1074 551 471 17 545 1096 1155